Contact an Account ManagerDrugs from Urine
Summary
Type: SPE
Application #: 301150
Catalog#: 730168 - SPE Cartridges, Chromabond, Drug, 3ml, PP, 200mg 50/pk
Detection:
Conditions: Solutions:
A) 0.1 M Na2HPO4: 14.2 g Na2HPO4 per litre H2O
B) 0.1 M NaH2PO4: 13.8 g NaH2PO4 per litre H2O
C) phosphate buffer pH 6: 1.7 g Na2HPO4 and 12.14 g NaH2PO4 dissolved in 1000
ml water and adjusted to pH 6 with A) or B)
D) 1.0 M acetic acid: 57.2 g glacial acetic acid per litre H2O
Column conditioning:
Carefully aspirate or force 3 ml methanol, 3 ml dist water and 1 ml phosphate
buffer (C) through the column
Sample application:
Slowly aspirate or force sample through the column (about 1 to 2 ml/min)
Column washing:
3 ml dist. water and then 1 ml 1.0 M acetic acid (D); dry column thoroughly by
applying vacuum for about 5 min
Aspirate or force 2 ml n-hexane through the column
Elution of acid and neutral drugs.
Slowly aspirate or force 3 ml n-hexane/ethyl acetate (1:1, v/v) through the
column, if necessary evaporate eluate in a stream of nitrogen and fill to 100
μl with ethyl acetate
Column washing:
Aspirate or force 3 ml methanol through the column, then dry for about 5 min
Elution of basic drugs.
Slowly aspirate or force 2 ml methanol/NH4OH 25% (98:2, v/v) through the
column; add 3 ml water and 250 μl chloroform to the eluate and mix well;
centrifuge for phase separation; analyse (lower) chloroform phase
Authors: Macherey-Nagel
Source: LC Department
Details
Legend: